full length trkb isoform  (Santa Cruz Biotechnology)

Journal: Cell Death & Disease

Article Title: Imbalance of p75 NTR /TrkB protein expression in Huntington's disease: implication for neuroprotective therapies

doi: 10.1038/cddis.2013.116

Figure Lengend Snippet: Imbalance between p75 NTR and TrkB expression in the striatum of R6/1 mice. ( a ) Representative immunoblots showing the levels of p75 NTR , TrkB and β -actin as a loading control in striatal extracts obtained from WT and R6/1 mice at 8 and 30 weeks of age. The histograms represent the relative levels of p75 NTR and TrkB expressed as percentage of wild-type values. Values are given as mean±S.E.M. of 5–6 independent samples. Data was analyzed by two-way ANOVA followed by Student's t -test. * P <0.05; ** P <0.01; *** P <0.001 respect to wild-type mice. ( b ) Representative immunohistochemical images depicting levels of p75 NTR staining in the striatum of WT and R6/1 mice at 30 weeks of age. The histogram represents the mean fluorescence intensity of p75 NTR (mean±S.E.M., n =3) expressed as percentage of wild-type levels. Data was analyzed by Student's t -test ** P <0.01 respect to wild-type mice. Scale bar, 10 μ m. ( c ) The histogram represents the mean fluorescence intensity of p75 NTR staining along rostral to caudal striatal sections from WT and R6/1 mice at 30 weeks of age expressed as percentage of wild-type values (mean±S.E.M. n =3). Data was analyzed by Student's t -test * P <0.05; ** P <0.01 respect to wild-type mice

Article Snippet: TrkB polyclonal antibody that recognizes the full-length TrkB isoform (sc-7268; C-tal epitope) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Western Blot, Control, Immunohistochemical staining, Staining, Fluorescence

Journal: Cell Death & Disease

Article Title: Imbalance of p75 NTR /TrkB protein expression in Huntington's disease: implication for neuroprotective therapies

doi: 10.1038/cddis.2013.116

Figure Lengend Snippet: p75 NTR and TrkB protein levels are also modified in the striatum of full-length Hdh Q111/111 mutant mice. ( a ) Representative immunoblots showing the levels of p75 NTR , TrkB and β -actin as a loading control in striatal extracts obtained from wild-type Hdh Q7/7 and mutant Hdh Q111/111 mice at 1, 2 and 12 months of age. The histograms represent the relative levels of p75 NTR and TrkB expressed as percentage of wild-type values. Values are given as mean±S.E.M. of 5–6 independent samples. Data was analyzed by two-way ANOVA followed by Student's t -test. * P <0.05; ** P <0.01; *** P <0.001 respect to wild-type mice. ( b ) (Upper panel) double immunostaining showing p75 NTR and DARPP32 in the striatum of wild-type ( Hdh Q7/7 ) and mutant ( Hdh Q111/Q111 ) mice at 12 months of age. Some cells are single-labeled for p75 NTR (arrows), whereas a high proportion of cells are double-labeled for p75 NTR and DARPP32 in both wild-type and HD mutant mice (arrowheads). (Middle panel) double immunostaining showing p75 NTR and parvalbumine staining in the striatum of mutant Hdh Q111/111 mice at 12 months of age. Most of the parvalbumine-positive neurons are also positive for p75 NTR . (Bottom panel) Double immunostaining showing p75 NTR and GFAP in the striatum of mutant ( Hdh Q111/111 ) mice at 12 months of age. p75 NTR and GFAP immunoreactivity demonstrates lack of colocalization. Scale Bar, 25 μ m

Article Snippet: TrkB polyclonal antibody that recognizes the full-length TrkB isoform (sc-7268; C-tal epitope) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Modification, Mutagenesis, Western Blot, Control, Double Immunostaining, Labeling, Staining

Journal: Cell Death & Disease

Article Title: Imbalance of p75 NTR /TrkB protein expression in Huntington's disease: implication for neuroprotective therapies

doi: 10.1038/cddis.2013.116

Figure Lengend Snippet: The putamen of HD patients shows altered p75 NTR and TrkB protein levels. ( a ) Human brain tissue from 10 control and 9 HD cases were analyzed by western blot. Immunoblots were probed with p75 NTR , TrkB, β -actin as loading control and NeuN and GFAP as neuronal and astroglial markers, respectively. Scatter plots display the relative levels of p75 NTR and TrkB normalized to actin, NeuN or GFAP. The horizontal line represents the normalized mean value in control and HD subjects. Data was analyzed using Student's t -test. ** P <0.01, *** P <0.001 compared with control. ( b ) Immunohistochemistry in caudate-putamen of HD brain (Vonsatel grade 1) showing extensive colocalization between DARPP32 (striatal neuronal marker) and p75 NTR . Nuclear staining was revealed by DAPI labeling to show that glia cells (arrows) are not positive for p75 NTR in contrast to neuronal cells (arrowheads). Scale bar, 10 μ m

Article Snippet: TrkB polyclonal antibody that recognizes the full-length TrkB isoform (sc-7268; C-tal epitope) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Control, Western Blot, Immunohistochemistry, Marker, Staining, Labeling